Tenth MaSC Workshop and Meeting
26 – 30 September 2022
Université de Bordeaux – Plateforme Protéome, Musée d’Aquitaine, Grand-Théâtre de Bordeaux
Bordeaux, France
Workshop, 26-28 September 2022:
Proteomics for cultural heritage and large data set analysis and treatment.
The three-day workshop provided a unique opportunity for participants to experience multidisciplinary training in advanced mass spectrometry techniques applied to protein characterization in artistic, archaeological and paleontological objects.
The workshop included both
(i) lectures led by local and international experts in proteomics, mass spectrometry, cultural heritage research, and heritage science and,
(ii) hands-on training in proteomics including sample preparation, MS acquisition, and data processing for the analysis of proteinaceous materials from objects of cultural heritage. The practical training included peptide mass fingerprint analysis (ZooMS), protein sequencing (LC-MS/MS) using bottom-up and top-down approaches, as well as mass spectrometry imaging. A set of complementary techniques was also used during the practical training (MALDI-TOF-TOF MS, MALDI/nanoESI-Orbitrap…).
The participants learned how to extract proteins from various type of cultural heritage objects and to prepare samples for analysis, addressing the very low sample amounts and the uniqueness of the material. They were shown how to optimize instrumental settings and methods to identify, quantify and/or characterize ancient proteins (structures, chemical modifications…). In addition, the participants gained insight into the development of the large datasets obtained using proteomics, their treatment, interpretation and storage (e.g. identification of proteins, identification of biological species, taxonomy, characterization of ancient protein damage…).
The workshop began with a welcome/orientation session the morning of September 26th.
The afternoon of Wednesday, September 28th was a wrap-up session in which the outcomes of the workshop were discussed and experiences were shared.
The practical hands-on workshop was limited to 20 participants due to laboratory space constraints.
Meeting, 29-30 September 2022:
The meeting was held at the Museum of Aquitaine on day one, and at the Grand Theatre of Bordeaux (Opera) on day two and comprised discussions and presentations on novel applications of chromatographic and mass spectrometric techniques to the study of art and cultural objects.
During these 2 days, pioneering researchers presented their most recent developments and achievements in the field of cultural heritage mass spectrometry, including technical and computational developments and applications.
The local organisers were Dr. Francesca Galluzzi, Dr. Aleksandra Popowich, and Prof. Caroline Tokarski.
MaSC 